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Electron microscopy of nanoparticle superlattice formation at a solid-liquid interface in non-polar liquids - Phys.org

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Electron microscopy of nanoparticle superlattice formation at a solid-liquid interface in non-polar liquids
Schematic representation of liquid phase electron microscopy (LP-EM) of oleylamine-capped AuNPs in nonpolar liquids. From left to right: Schematic of the assembled liquid cell. A droplet of AuNPs is placed on a microchip with a thin SiN membrane window and containing a spacer of 150-nm thickness, after which a liquid cell is assembled using a second microchip. Images are obtained by scanning the electron beam over the sample and recording transmitted electrons, whereby the liquid is protected from evaporation by the SiN membrane window. Data of self-assembled nanoparticles are then analyzed. Credit: Science Advances, doi: 10.1126/sciadv.aba1404

Nanoparticle superlattice films that form at the solid-liquid interface are important for mesoscale materials but are challenging to analyze on the onset of formation at a solid-liquid interface. In a new report on Science Advances, E. Cepeda-Perez and a research team in materials, physics and chemistry in Germany studied the early stages of nanoparticle assembly at solid-liquid interfaces using liquid-phase electron microscopy. They observed oleylamine-stabilized gold nanoparticles to spontaneously form thin layers on a silicon nitride membrane window of the liquid enclosure. In the first monolayer, the assembly maintained dense packings of hexagonal symmetry independent of the nonpolar solvent type. The second layer displayed geometries ranging from dense packing in a hexagonal honeycomb structure to quasi-crystalline particle arrangements—based on the dielectric constant of the liquid. The complex structures made of weaker interactions remained preserved, while the surface remained immersed in liquid. By fine-tuning the properties of materials involved in nanoparticle superlattice formation, Cepeda-Perez et al. controlled the three-dimensional (3-D) geometry of a superlattice, including quasi-crystals (a new state of matter).

Nanoparticles that are densely packed into two or three dimensions can form regular arrays of nanoparticle superlattices. For example, semiconductor particle superlattices can act as "meta" semiconductors when doped with particles to form new mesoscale materials, while plasmonic particles in dense superlattices can couple to form collective modes with angle-dependent and tunable wavelength responses. Large electric fields can occur between such particles for surface-enhanced Raman spectroscopy. Superlattices can be developed at liquid-liquid, gas-liquid and solid-liquid interfaces, where static and dynamic interactions between particle-substrate, particle-particle and particle-liquid interactions can dictate the structure of superlattices. However, it remains difficult to predict such structures in advance. For example, simulating the assembly of superlattices at multiple stages is not yet possible, with very little in-lab real-space data available for modeling. It is therefore challenging to gather experimental insights on fundamental mechanisms of formation.

Electron microscopy of nanoparticle superlattice formation at a solid-liquid interface in non-polar liquids
AuNPs films formed in liquid at the interface with a SiN membrane. Exemplary scanning transmission electron microscopy (STEM) images observed in different nonpolar liquids: (A) hexadecane (liquid thickness lsolv = 3.9 μm, electron flux D = 1.3 e−/sÅ2), (B) octane (lsolv = 6.9 μm, D = 0.3 e−/sÅ2), (C) cyclohexane (lsolv = 2.3 μm, D = 0.3 e−/sÅ2), and (D) toluene (lsolv = 1.0 μm, D = 0.3 e−/sÅ2). Credit: Science Advances, doi: 10.1126/sciadv.aba1404

In this work, Cepeda-Perez et al. directly imaged superlattices of gold (AuNPs) suspended in different nonpolar solvents using liquid-phase electron microscopy (LP-EM). The AuNPs in the first layer above a surface, strongly interacted with the substrate and each other. As a superstructure assembled layer-by-layer, the surface interactions diminished, and the patterns encountered weaker forces between nanoparticles mediated by the properties of the surrounding liquid. The team studied transitions from particle-surface interactions to weaker interparticle interactions by examining patterns of a monolayer and a double layer, under different solvent conditions. To study the origin of superlattices they examined a single type of oleylamine-coated AuNPs assembled on a silicon nitride (SiN) coated membrane. To understand interactions between AuNPs and the substrate, the scientists changed the solvent and used scanning transmission electron microscopy (STEM) to obtain the highest spatial resolution possible with the lowest possible electron flux.

The team subjected the SiN (silicon nitride) membranes to an oxidizing plasma to induce surface polarity directly before loading the sample. Then using liquid-phase electron microscopy they observed the gold nanoparticles (AuNPs) located at the solid-liquid interface. They tested four different nonpolar liquids with different dielectric constants to vary the range of interactions. Particle-substrate interactions attracted the nanoparticles from the liquid to the SiN surface to form a thin film. The strong and weak interactions caused dense packings that were only weakly affected by the choice of solvent. The overall densities were highest for the linear-chain solvents.

Electron microscopy of nanoparticle superlattice formation at a solid-liquid interface in non-polar liquids
Single layers of AuNP at the solid-liquid interface in hexadecane. (A) STEM image of a single layer of AuNPs. The inset corresponds to the FFT: lsolv = 3.9 μm, D = 5.0 e−/sÅ2. (B) Plot of the gray value versus position acquired for the red line shown in (A). Credit: Science Advances, doi: 10.1126/sciadv.aba1404

When imaging the AuNP layer in hexadecane solvent, the team observed brighter and fainter spots to indicate the presence of AuNPs on top of a monolayer—alongside three discrete levels from the signal of the image. The observed patterns remained stable even after they recorded images of the sample area in a time-lapse. Comparatively, cyclohexane non-polar organic solvents led to a dense, hexagonal top layer and the team noted structures with a hexagonal base and rhombitrihexagonal (characterized by one triangle, two parallelograms, and one hexagon on each vertex) tiling. The samples also showed patches of quasi-crystalline nature. Compared to the first layer of particles, the team observed weaker bonding of particles in the second layer, they credited reduced particle packing density to the decay of particle-substrate interaction potential.

Electron microscopy of nanoparticle superlattice formation at a solid-liquid interface in non-polar liquids
Crystal and quasi-crystal AuNP structures self-assembled at a solid-liquid interface. (A) Hexagonal lattice observed for AuNPs in cyclohexane: lsolv = 0.5 μm, D = 30.4 e−/sÅ2. (B) AuNPs in toluene arranged in a rhombitrihexagonal pattern (colored area): lsolv = 0.8 μm, D = 30.4 e−/sÅ2. (C) Schematic of rhombitrihexagonal tiles. The dimensions found for the triangles, parallelograms, and hexagons (each side) were 8.3 nm, 8.3 nm by 10 nm, and 10 nm, respectively. (D) AuNPs in toluene arranged in a non–self-similar modification of the BMQC (colored area) (lsolv = 0.8 μm, D = 30.4 e−/sÅ2), (E) dimensions of the non–self-similar BMHC, and (F) its tiles and fundamental dodecagon symmetry. Credit: Science Advances, doi: 10.1126/sciadv.aba1404

In this way, E. Cepeda-Perez and colleagues showed that solvents with high dielectric constants reduced the packing densities and allowed the formation of quasi-crystalline structures. The results were consistent with the hypothesis of the study on substrate-induced self-assembly and particle-substrate attraction. The LP-EM data explained the mechanisms behind the formation of self-assembled superlattices of at a solid-liquid interface. The outcomes led to quasi-crystalline particle arrangements, which depended on the strength of the dielectric constant of the liquid in the setup. Based on the results, the 3-D geometry of a superlattice including complex quasi-crystalline structures can be fine-tuned by manipulating the properties of the involved liquid, nanoparticles, and interface materials.

Electron microscopy of nanoparticle superlattice formation at a solid-liquid interface in non-polar liquids
Time-lapse STEM image series of a self-assembled multi-layer structure in toluene. The first image of this series eventually arranged into a rhombitrihexagonal pattern. The experimental conditions include lsolv =0.8 μm, D = 30.4 e−/sÅ2. Credit: Science Advances, doi: 10.1126/sciadv.aba1404

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More information: E. Cepeda-Perez et al. Electron microscopy of nanoparticle superlattice formation at a solid-liquid interface in nonpolar liquids, Science Advances (2020). DOI: 10.1126/sciadv.aba1404

Angang Dong et al. Binary nanocrystal superlattice membranes self-assembled at the liquid–air interface, Nature (2010). DOI: 10.1038/nature09188

Eiji Abe et al. Quasicrystals as cluster aggregates, Nature Materials (2004). DOI: 10.1038/nmat1244

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